Journal: Bioactive Materials

Article Title: A self-locking conductive cardiac patch for immediate electrical integration with infarcted rat myocardium

doi: 10.1016/j.bioactmat.2025.10.045

Figure Lengend Snippet: The effects of patches on angiogenesis and inflammation in infarcted tissues 4 weeks post-MI. a) Immunofluorescence staining of α-smooth muscle actin (α-SMA, a vascular protein marker, green) and von Willebrand factor (vWF, an endothelial marker, red) of the infarct region in the MI, BMN-P, BMN-CP and CBMN-CP groups. b, c) Quantitative analysis of α-SMA (b) and vWF (c) in different groups based on fluorescent staining images (n = 4). d) Representative immunofluorescence staining of the infarct region in different groups for CD86 (green) and CD206 (red). e, f) Fluorescence intensity statistics of CD86 (e) and CD206 (f) in different groups based on fluorescent staining images (n = 4). Nuclei are stained blue with DAPI. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001.

Article Snippet: The tissue sections were then incubated with the following primary antibodies: rabbit Connexin 43 primary antibody (BOSTER, BA1727, 1:200, China), mouse α-actinin primary antibody (Abcam, AB9465, 1:200, UK), mouse α-SMA primary antibody (Wuhan Sanying, 67735-1-IG, 1:400, China), rabbit vWF primary antibody (Wuhan Sanying, 27186-1-AP, 1:300, China), mouse CD86 primary antibody (BOSTER, BA4121, 1:100, China) and rabbit CD206 primary antibody (Wuhan Sanying, 18704-1-AP, 1:400, China).

Techniques: Immunofluorescence, Staining, Marker, Fluorescence

Journal: Frontiers in Immunology

Article Title: Repetitive transcranial magnetic stimulation alleviates neuropathic pain via microglial polarization by modulating the METTL3/NMDAR2B/NLRP3 pathway

doi: 10.3389/fimmu.2025.1666920

Figure Lengend Snippet: Effects of rTMS on neuroinflammation and the M1/M2 polarization state of microglia in vivo . (A–C) Elisa was used to examine the expression levels of IL-6, TNF-α and IL-10 expression level. (D–F) Real-time qRT-PCR analysis of the relative mRNA expression of TMEM119 , iNOS , and Arg1 in the DLPFC. (G, H) Immunofluorescence staining of Iba-1, M1-type (CD86) and M2-type (CD206) markers in the left PFC region. (I, J) Proportion of positive cells for target proteins. The number of samples in each group is 3. The results was analyzed with one-way ANOVA followed by Tukey’s post-hoc test, *p < 0.05, **P < 0.01 and ***P < 0.001.

Article Snippet: Primary antibodies against CD86 (1:100; bs-1035r; Bioss), CD206 (1:100; #24595; CST) and Iba-1(1:100, ab283319, Abcam, Cambridge, UK)were applied, and the sections were incubated overnight at 4 °C.

Techniques: In Vivo, Enzyme-linked Immunosorbent Assay, Expressing, Quantitative RT-PCR, Immunofluorescence, Staining

Journal: Frontiers in Immunology

Article Title: Repetitive transcranial magnetic stimulation alleviates neuropathic pain via microglial polarization by modulating the METTL3/NMDAR2B/NLRP3 pathway

doi: 10.3389/fimmu.2025.1666920

Figure Lengend Snippet: Effects of rTMS on neuroinflammation and the M1/M2 polarization state of microglia in vitro . (A–D) Elisa was used to examine the expression levels of NLRP3, IL-6, TNF-α and IL-10 expression level. (E–G) Immunofluorescence staining of Iba-1, CD86, CD206. (H–J) Proportion of positive cells for target proteins. The number of samples in each group was 3. The results was analyzed with one-way ANOVA followed by Tukey’s post-hoc test, *p < 0.05, **P < 0.01 and ****P < 0.0001.

Article Snippet: Primary antibodies against CD86 (1:100; bs-1035r; Bioss), CD206 (1:100; #24595; CST) and Iba-1(1:100, ab283319, Abcam, Cambridge, UK)were applied, and the sections were incubated overnight at 4 °C.

Techniques: In Vitro, Enzyme-linked Immunosorbent Assay, Expressing, Immunofluorescence, Staining

Journal: Frontiers in Immunology

Article Title: Repetitive transcranial magnetic stimulation alleviates neuropathic pain via microglial polarization by modulating the METTL3/NMDAR2B/NLRP3 pathway

doi: 10.3389/fimmu.2025.1666920

Figure Lengend Snippet: The roles of METTL3 and NMDAR2B in the regulation of microglial cell polarization by magnetic stimulation in vitro . (A–C, G–I) Immunofluorescence staining of Iba-1, CD86 and CD206. (D–F, J–L) Proportion of positive cells for target proteins. The number of samples in each group was 3. The results was analyzed with one-way ANOVA followed by Tukey’s post-hoc test, *p < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.

Article Snippet: Primary antibodies against CD86 (1:100; bs-1035r; Bioss), CD206 (1:100; #24595; CST) and Iba-1(1:100, ab283319, Abcam, Cambridge, UK)were applied, and the sections were incubated overnight at 4 °C.

Techniques: In Vitro, Immunofluorescence, Staining

Journal: Materials Today Bio

Article Title: Vitamin C–functionalized copper nanozymes for treating drug-resistant intracellular infections and hyperinflammation

doi: 10.1016/j.mtbio.2025.102348

Figure Lengend Snippet: ROS- s cavenging and a nti- i nflammatory p roperties of CVs. (A). CLSM images showing ROS green fluorescent levels in RAW264.7 cells with DCFH-DA probe. Scale bar, 50 μm. (B). Representative images of flow cytometry detecting ROS with various groups in RAW264.7 cells. (C). Quantitative analysis of the mean fluorescence intensity of ROS depicted in B. (n = 3, mean ± s.d.). (D). Representative images of flow cytometry detecting apoptosis rates of RAW264.7 cells in different groups. (E). Quantitative analysis of the MFI of apoptosis rates depicted in D. (n = 3, mean ± s.d.). (F). Representative immunofluorescence images of CD86 in various groups. Scale bar, 50 μm. (G). Representative images of flow cytometry detecting CD86 with various groups in RAW264.7 cells. (H). Quantitative analysis of the MFI of CD86 depicted in G. (n = 3, mean ± s.d.). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: CD86 was detected using an anti-CD86 primary antibody (Proteintech, China), while nuclei were counterstained with DAPI.

Techniques: Flow Cytometry, Fluorescence, Immunofluorescence

Journal: Journal of Clinical and Translational Hepatology

Article Title: Arctigenin Prevents Metabolic Dysfunction-associated Steatohepatitis by Inhibiting NLRP3/GSDMD-N Axis in Macrophages

doi: 10.14218/JCTH.2025.00141

Figure Lengend Snippet: (A) Chemical structure of ATG. (B) Target prediction of ATG. (C) Molecular targets associated with MAFLD. (D) Intersection of ATG-specific and MAFLD-related targets. (E) The PPI network of intersection targets between ATG and MAFLD-related targets. (F) GO enrichment analysis of the intersected targets. (G–H) Enrichment of KEGG pathway (G) and Reactome pathway (H) of ATG’s targets. (I–J) Immunofluorescence analysis of the colocalization of NLRP3 and CD86 in mice liver from four groups (scale bar: 20 µm), staining intensity was quantified, and colocation analysis was performed. (K) NLRP3 with ATG bound to the cavity. (L) Western blot of CETSA experiment to further confirm the interaction between NLRP3 and ATG in RAW264.7 cells, the temperature ranges from 49°C to 61°C, with relative band intensity normalized to 49°C. Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. ATG, arctigenin; MAFLD, metabolic dysfunction-associated fatty liver disease; PPI, protein-protein interaction; KEGG, Kyoto Encyclopedia of Genes and Genomes; NLRP3, NLR family pyrin domain containing 3; SEM, standard error of the mean.

Article Snippet: Primary antibodies against CD86 (19589, Cell Signaling Technology, CST®; 1:400 in 3% BSA) and α-SMA (ab124964, Abcam®; 1:2,000 in 3% BSA) were incubated overnight.

Techniques: Immunofluorescence, Staining, Western Blot